Sequencing Estimator

Sequencing Estimator

Rhett M. Rautsaw

github.com/RhettRautsaw/SequencingEstimator

This application is designed to help you choose the right sequencing platform and estimate costs. The app currently provides space for Whole-Genome Sequencing (WGS), RNA-seq, Whole-Genome Bisulfite Sequencing (WGBS), ATAC-Seq, and ddRAD-Seq. However, given desired coverage or number of reads (in million), the fields could be used any type of sequencing. Most information was obtained from: Illumina The recommended number of reads and coverage are default input for all options

Genome Size (Gb):

Not required for RNA-Seq, ATAC-Seq, ddRAD-Seq, or any sequencing type based on sequencing a specific number or reads rather than coverage.

Read Length:

Read length is not applicable for PacBio sequencing and will be included regardless. Illumina sequencers will be filtered by which are applicable with the chosen read length.

WGS Samples:

WGS Coverage:

RNA-Seq Samples:

RNA-Seq reads per sample (in millions):

WGBS Samples:

WGBS Coverage:

ATAC-Seq Samples:

ATAC-Seq reads per sample (in millions):

ddRAD-Seq Samples:

ddRAD-Seq reads per sample (in millions):

If you have updated information on sequencing platforms or costs, you can download an example of the necessary data, update it, and re-upload it here for your needs. Download

Choose CSV File

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